Intshayelelo
I-Cholera Prikemics, ibangelwa yi-v.cholerae serotype O1, Qhubeka ubeIsifo esitshabalalisayo sezinto ezinkulu zehlabathi kwizinto ezininzi ezikhulayoamazwe. Ikliniki, i-cholera inokuvela kwi-Aymmpumatic colodisozer ukuya kwiiswekile enkulu enelahleko enkulu ye-fluid, ekhokelela ekuphelisweni, i-electrolyteukuphazamiseka, nokufa. V. I-cholerae o1 ibangela le suarrhea yemvelisoIkholoni yamathumbu amancinci kunye nokuveliswa kwe-cholera enobuninzi be-cholera Toxin,Ngenxa yokuphazamiseka kweklinikhi kunye notyholo lweKliniki yeKholera, kuya kuba lulekeUkuchonga ngokukhawuleza kunokwenzeka ukuba ingaba ilungu lezinto ezivela kwisigulanaI-huarhea emanzi inomdla kwi-v.cholera o1. Ukukhawuleza, i-Dlula kwaye inokuthenjwaIndlela yokufumana i-v.cholerae O1 lixabiso elikhulu kunyango ekulawuleniEsi sifo kunye namagosa ezempilo esidlangalaleni kwiZiko loLawulo.

Umgaqo
I-Vibrio Cholerae O1 I-Antign Antign Traid Food (i-Fieds) iqaphelele iVibrioI-cholerae o1 ngokutolikwa kwemveliso yophuhliso lwemibala ngaphakathihlula. I-Anti- Vibrio Cholerae O1 Antibodies ayifakwanga kwingingqi yovavanyo yei-membrane. Ngexesha lovavanyo, i-propt ye-anti- vibrio cholerae o1Ii-antibodies zidityaniswe kwibala lemibala kwaye ichazwe kwi-pad yesampulu yeUvavanyo. Umxube emva koko fudukela kwi-membrane ngesenzo se-capillary kwayeUkunxibelelana nee-regents kwi-membrane. Ukuba kukho i-vibrio ye-vibrio ye-O1Kwinqanaba, ibhendi enemibala iya kwenza kwingingqi yovavanyo ye-membrane. Iubukho beli band incwecwel ibonisa isiphumo esihle, ngelixa ukungabikho kwayoibonisa isiphumo esibi. Ukubonakala kweqela elinemibala kulawuloUmda usebenza njengolawulo lwenkqubo, ebonisa ukuba ivolumu efanelekileyo yeI-Specimen yongezwa kwaye i-membrane iwikeki yenzekile.

UKULUMKELA
• Kwingcali kwi-vitro yokusetyenziswa kuphela.
• Musa ukusebenzisa emva komhla wokuphelelwa oboniswe kwiphakheji. SukusebenzisaUvavanyo ukuba ibhedi ye-foil yonakalisiwe. Sukuyifumana kwakhona iimvavanyo.
• Le khiti inemveliso yemvelaphi yezilwanyana. Ulwazi oluqinisekisiweyo lweImvelaphi kunye / okanye imeko ye-STATAT yezilwanyana ayiqinisekanga ngokupheleleyoUkungabikho kweearhente ze-pithogenic ezinikiweyo. Ke ngoko,Kucetyiswa ukuba ezi mveliso zinyangwa njengokusuleleka, kwayeIphathwe ngokubona amanyathelo okhuseleko okhuseleko (umzekelo, musa ukufumanisa okanye ungangeni).
• Kulumkele ukungcoliseka kwesampulu ngokusebenzisa inqaku elitshaIsikhongozeli sokuqokelela kwinqaku ngalinye elifunyenweyo.
• Funda yonke le nkqubo kwangaphambili ngaphambi kovavanyo.
• Musa ukutya, usele okanye umsi nakweyiphi na indawo apho iisampulu kunye neekhitshi ziphathwa.Phatha zonke iimodeli ngokungathi ziqulathe iiarhente zosulelo. JongaUKULUNGISELELA UKUQHUTYELWA KWEEMPAHLA ZE-Microbiological kuyo yonke le nkqubo kwayeLandela iinkqubo ezisemgangathweni zokulahlwa ngokuchanekileyo. Nxiba ukukhuselaImpahla enjengeengubo zelebhu, iiglavu ezilahlekileyo kunye neeglavu ezihlaselayo kunye ne-Wighcimes yokhuseleko lwamehlo ziyathandeka.
• I-buffer ye-chalution iqulathe i-sodium Atside, enokuphendula ngokukhokelaokanye i-pliper plist ukwenza i-Azas efihlakeleyo. Xa ulahlaye-buffer ye-capimen buffer okanye iisampulu ezikhutshelweyo, zihlala zigungxulwa ngokutshaubungakanani bamanzi ukuthintela ukwakha i-Azeide.
• Musa ukuguqula okanye ukuxuba iirejista ukusuka kwiindawo ezahlukeneyo.
• Ukufuma kunye nobushushu kunokuchaphazela kakubi iziphumo.
• Izixhobo zokuvavanya ezisetyenzisiweyo kufuneka zilahliwe ngokwemigaqo yendawo.