Rotavirus Antigen Rapid Test
INTSHAYELELO
I-Rotavirus yeyona arhente ixhaphakileyo ejongene ne-acute gastroenteritis, ngakumbi kubantwana abancinci.Ukufunyanwa kwayo ngo-1973 kunye nonxulumano lwayo ne-infantile gastro-enteritis imele ukuqhubela phambili okubaluleke kakhulu kuphononongo lwe-gastroenteritis engabangelwa lusulelo olubukhali lwebhaktiriya.I-Rotavirus isasazwa nge-oral-fecal route kunye nexesha lokufukamela iintsuku ezi-1-3.Nangona iisampuli eziqokelelwe phakathi komhla wesibini kunye nowesihlanu wokugula zilungele ukufunyanwa kwe-antigen, i-rotavirus isenokufumaneka ngelixa isifo sohudo siqhubeka.I-Rotaviral gastroenteritis inokubangela ukusweleka kwabantu abasengozini njengeentsana, abantu abadala kunye nezigulana ezibuthathaka.Kwimozulu epholileyo, ukusuleleka kwe-rotavirus kwenzeka ngokukodwa kwiinyanga zasebusika.Kuye kwanikelwa ingxelo ngoobhubhani kunye noobhubhani abachaphazela abantu abaliwaka.Kubantwana ababhedlele esibhedlele abaphethwe yi-acute enteric disease, ukuya kuthi ga kwi-50% yemizekelo ehlalutyiweyo yayine-rotavirus.Iintsholongwane ziphindaphinda kwi
iseli nucleus kwaye ithande ukuba umkhosi weentlobo-specific uvelisa uphawu cytopathic effect (CPE).Ngenxa yokuba i-rotavirus inzima kakhulu kwinkcubeko, akuqhelekanga ukusebenzisa i-virus yodwa ekuxilongweni kwezifo.Endaweni yoko, kuye kwaphuhliswa iindlela ezahlukeneyo zokubona i-rotavirus kwilindle.
UMGAQO
Isixhobo soVavanyo oluKhawulezayo lwe-Rotavirus (Iindle) sibona i-rotavirus ngokutolika okubonakalayo kophuhliso lombala kumgca wangaphakathi.I-Anti-rotavirus antibodies ayishukumi kwindawo yovavanyo ye-membrane.Ngexesha lokuvavanya, umzekelo
iphendula ngee-anti-rotavirus antibodies ezidityaniswe kumasuntswana anemibala kwaye ziqatywe ngaphambili kwisampulu yovavanyo.Umxube ke ufuduka kwi-membrane ngesenzo se-capillary kwaye udibanise ne-reagents kwi-membrane.Ukuba kukho
i-rotavirus eyaneleyo kwi-specimen, ibhande elinemibala liya kwenza kwindawo yokuvavanya kwi-membrane.Ubukho beli bhanti elinemibala libonisa umphumo omuhle, ngelixa ukungabikho kwawo kubonisa umphumo ombi.Ukubonakala kwebhanti enemibala kwi
ummandla wokulawula usebenza njengolawulo lwenkqubo, ebonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.
AMANQAKU EKIT
| Izixhobo zovavanyo ezipakishwe ngumntu ngamnye | Isixhobo ngasinye sinomgca onama-conjugates anemibala kunye nee-reagents ezisebenzayo ezifakwe ngaphambili kwiindawo ezihambelanayo. |
| Isampulu ityhubhu yodilution ene-buffer | I-0.1 M I-Phosphate buffered saline (PBS) kunye ne-0.02% ye-sodium azide. |
| Iipayipi ezilahlayo | Ukuqokelela imizekelo yolwelo |
| Faka iphakheji | Ngemiyalelo yokusebenza |
IZINTO EZIFUNEKAYO KODWA AKUNIKEZWE
| Isibali-xesha | Ukusetyenziswa kwexesha |
| Centrifuge | Ukunyanga imizekelo kwiimeko ezizodwa |
Iziqinisekiso
