I-rotavirus antign yovavanyo olukhawulezayo



Intshayelelo
I-Rotavirus yeyona arhente eqhelekileyo enoxanduva lokuxhasa i-jastroenteritis, ikakhulu kubantwana abancinci. Ukunxulumana kwawo ngo-1973 kwaye ukunxulumana ne-gestaile ye-gestaitis yayimela ukuqhubela phambili kakhulu kufundo lwe-Gastroenteritis abubangelwa lusulelo lwentsholongwane. I-Rotavirus idluliselwa yindlela yomlomo ye-off-fecal enexesha leentsuku ezili-1-3. Nangona ii-mores eziqokelelwe ngaphakathi kosuku lwesibini nolwesihlanu zilungele ukuhlolwa kwe-Antigden, uRotavirus usenokufunyanwa ngelixa urhudo uqhubeka. I-rotaviral gestroenteritis inokubangela ukusweleka kwabantu abasemngciphekweni onjengeentsana, abadala kunye nabaguli abakhulileyo. Kwimozulu yezulu, usulelo lweRotavirus lwenzeka ikakhulu kwiinyanga zobusika. I-endemics kunye ne-piidemics echaphazela amawaka abantu ziye zaxelwa. Ngoonyana basebhedlele esibhedlele abaphethwe sisifo se-ARTEC, ukuya kuthi ga kwi-50% yeesampulu ezihlalutyweyo zazilungile kwiRotavirus. Iintsholongwane ziyaziphinda
I-nucleus yeseli kwaye ithande ukuba i-bers i-orting ivelisa isiphumo se-CyPototic (CPE). Ngenxa yokuba uRotavirus kunzima kakhulu kwinkcubeko, oko akuqhelekanga ukusebenzisa i-virus ekufumaneni kosulelo. Endaweni yoko, iindlela ezahlukeneyo zobuchwephesha ziphuhlisiwe ukuba zibone i-rotavirus kwimithombo.
Umgaqo
Isixhobo sovavanyo lweRotavirus (i-feeds) ifumana iRotavirus ngokutolikwa koMbala woPhuhliso lwangaphakathi. I-anti-rotavirus antibodies ayifakwanga kwingingqi yovavanyo ye-membrane. Ngexesha lovavanyo, inqaku
I-projeks nge-anti-rotavirus antibodies idityaniswe kwibala elinemibala kwaye ichazwe kwiPad yovavanyo lovavanyo. Umxube emva koko fudukela kwi-membrane ngezenzo ze-capillary kwaye inxibelelana nee-regents kwi-membrane. Ukuba ikhona
I-rotavirus eyaneleyo kwi-Special, ibhendi enemibala iya kwenza kumda wovavanyo lwe-membrane. Ubukho beli band yebala libonisa isiphumo esihle, ngelixa ukungabikho kwayo kubonisa iziphumo ezimbi. Imbonakalo yeqela lemibala kwi
Umda wolawulo usebenza njengokulawulwa kwenkqubo, ebonisa ukuba ivolumu efanelekileyo ye-Specimen kwaye iwikene ye-membrane yenzekile.
Izinto zekhithi
Izixhobo zovavanyo oluqhelekileyo | Isixhobo ngasinye siqukethe umtya onemibala enombala kunye nokwenza izinto ezisebenzayo ezihlanganisiweyo kwimimandla ehambelanayo. |
I-capensimens ityhubhu ye-buffer | I-0.1 M phosphate ye-phosphate ye-saline (PBs) kunye ne-0.02% ye-sodium aze. |
Imibhobho ye-Pipettes | Ukuqokelela iimodeli zolwelo |
Iphakheji | Imiyalelo yokusebenza |
Izinto ezifunekayo kodwa azibonelelwanga
Ixesha | Ukusetyenziswa kwexesha |
I-Centrifuge | Ngonyango lwe-capemens kwiimeko ezizodwa |