Uvavanyo olukhawulezayo olukhawulezileyo
Ukusetyenziswa kwenjongo
I-strongstep®Uvavanyo lwePront luyitolikiweyo, ukuchaza amagqabantshintshi ngenjongo yokufunyanwa kwe-Igfbp-1 ukusuka kwi-Amniotic fluid kwi-Viginal. Uvavanyo lwenzelwe ukusetyenziswa ngobungcali ukunceda ekufumanisweni kwendlela yokuqhekeza i-membranes (i-ROM) kubafazi abakhulelweyo.
Intshayelelo
Ukugxininiswa kwe-IGFBP-1 I-IGFBP-1 ayisoloko ikho kwilungu lobufazi, kodwa emva kokugqabhuka kwamabala, i-amniotic fluid enoxinzelelo oluphezulu lwe-IGFBP-1 yemigangatho ye-Viginal. Uvavanyo lwe-certstep teststep ®, ukufihlakala kwe-Viginal kuthathwa nge-wilyester polyester swab kwaye i-starimen ikhutshwe kwisisombululo sokukhuphela. Ubukho be-IGFBP-1 kwisisombululo kufunyanwa usebenzisa isixhobo sovavanyo ngokukhawuleza.
Umgaqo
I-strongstep®Uvavanyo lwepront lusebenzisa imizobo ye-itunochromatographic, i-capillary yokuhambahamba. Inkqubo yovavanyo ifuna ukulungisa i-igfbp-1 ukusuka kwi-vablinal swab ngokuxuba i-swab kwi-buffer yesampulu. Emva koko i-buffer exutywe yongezwa kwimodeli yekhasethi yovavanyo kakuhle kunye nomxube wokufuduka ecaleni kwe-membrane. Ukuba i-Igfbp-1 ikho kwisampulu, iya kwenza ubunzima nge-anti- 1 i-anticturd idityaniswe kwibala elinemibala. Ubunzima buya kuthi emva koko buboshwe yi-Anti- 1 i-antichedy inamathele kwi-nitrocelsulose membrane. Ukubonakala komgca wovavanyo obonakalayo kunye nomgca wokulawula uya kubonisa isiphumo esifanelekileyo.
Izinto zekhithi
| 20 Nganye nganyeackIzixhobo zovavanyo lwe-ED | Isixhobo ngasinye siqukethe umtya onemibala enombala kunye nokwenza izinto ezisebenzayo ezihlanganisiweyo kwimimandla ehambelanayo. |
| 2UkukhutshwaI-Buffer Vial | I-0.1 M phosphate ye-phosphate ye-saline (PBs) kunye ne-0.02% ye-sodium aze. |
| I-1 yoLawulo lweSwab (ngesicelo kuphela) | Iqulethe i-Igfbp-1 kunye neSodium Atside. Ulawulo lwangaphandle. |
| I-1 ye-swab (ngesicelo kuphela) | Ungabinayo i-Igfbp-1. Ulawulo lwangaphandle. |
| 20 Ukukhupha iityhubhu | Ukusetyenziswa kwengqondo yokusetyenziswa. |
| 1 Indawo yokusebenza | Indawo yokubamba i-vials ye-buffer kunye neetyhubhu. |
| 1 Iphakheji | Uqeqesho lokusebenza. |
Izinto ezifunekayo kodwa azibonelelwanga
| Ixesha | Ukusetyenziswa kwexesha. |
UKULUMKELA
■ Ingcali kwi-vitro yokusetyenziswa kuphela.
■ Musa ukusebenzisa emva komhla wokuphelelwa oboniswe kwiphakheji. Sukusebenzisa uvavanyo ukuba ibhedi yayo yonakalisiwe. Sukuyifumana kwakhona iimvavanyo.
■ Le khiti inemveliso yemvelaphi yezilwanyana. Ulwazi oluqinisekisiweyo lwemvelaphi kunye / okanye ilizwe lezilwanyana aliqinisekisi ngokupheleleyo ukungabikho kweearhente ze-pithogenic ezinikiweyo. Ke ngoko, kucetyiswa ukuba ezi mveliso zinyangwa njengokungasuleleki, kwaye zisingathethwa zizinto zokhuseleko eziqhelekileyo (musa ukufaka okanye i-inhale).
■ Khusela ungcoliseko olunqamlezileyo lwemodeli ngokusebenzisa isikhongozelo esitsha sengqokelela kwinqaku ngalinye elifunyenweyo.
■ funda yonke inkqubo ngononophelo ngaphambi kokwenza naluphi na uvavanyo.
■ Ungayityi, usele okanye umsi kwindawo apho iisampulu kunye neekhitshi ziphathwa. Phatha zonke iimodeli ngokungathi ziqulathe iiarhente zosulelo. Observe established precautions against microbiological hazards throughout the procedure and follow the standard procedures for proper disposal of specimens. Nxiba impahla yokhuselo enjengeengubo zelebhu, iiglavu ezihlangeneyo kunye nokukhuseleka kwamehlo xa kuthathwa iisampulu.
■ Musa ukungahambelani okanye udibanise iirejista ukusuka kwiindawo ezahlukeneyo. Sukudibanisa isisombululo sebhotile yebhotile.
■ Ukuqina kunye nobushushu kunokubachaphazela kakubi iziphumo.
■ Xa i-Assay Sgramme igqityiwe, ilahla i-swabs ngononophelo emva kokuziphindezela nge-121 ° C ubuncinci imizuzu engama-20. Ngenye indlela, banokuthi baphathwe nge-0.5% ye-sodium hypochloride (okanye indlu-i-veach) iyure enye ngaphambi kokulahlwa. Izixhobo zokuvavanya ezisetyenzisiweyo kufuneka zilahliwe ngokungqinelana nendawo yengingqi, yelizwe kunye / okanye imigaqo ye-Federal.
■ Musa ukusebenzisa ibhulegy ye-cytology kunye nezigulana ezikhulelweyo.
Ukugcinwa kunye nokuzinza
■ Ikhithi kufuneka igcinwe kwi-2-30 ° C kude kube ngumhla wokuphelelwa kwingxowa etywiniweyo.
■ Uvavanyo kufuneka luhlale kwi-pouch etywiniweyo de isebenzise.
■ Musa ukundikhupha.
■ Ixhala kufuneka lithathwe ukukhusela izinto kule khithi kungcoliseko. Sukusebenzisa ukuba kukho ubungqina bongcoliseko lwe-rotial okanye i-puciption. Ungcoliseko lwebhayoloji lokusasaza izixhobo, izikhongozeli okanye iirejints zingakhokelela kwiziphumo zobuxoki.
Ukuqokelelwa kwengqokelela kunye nokugcina
Sebenzisa kuphela i-dacron okanye iRayon ikhuphe i-stabs ye-suble enezixhobo zeplastiki. Kucetyiswa ukusebenzisa i-swab ebonelelwe yi-Kits Peecter (i-swabs ayikho kule khithi, kulwazi loku-odola, nceda tsalela uMvelisi okanye inombolo yengingqi, inombolo ye-Cataloge yi-207000). I-Swabs evela kwabanye abathengisi akazange aqinisekiswe. I-swabs ngeingcebiso zomqhaphu okanye ii-shaftts zenkuni azicetyiswa.
■ Isampulu ifunyanwa kusetyenziswa i-polyester polyester polyester swab. Isampulu kufuneka iqokelelwe ngaphambi kokwenza uviwo lwedijithali kunye / okanye i-transvaginal iltrasound. Thatha inkathalo ukuba ungachukumisi nantoni na ngeSwab ngaphambi kokuba uthathe isampula. Faka ngononophelo inqaku le-swab kwilungu lobufazi ukuya kwi-posterix fortix de kufikeleleke. Ngenye indlela isampulu inokuthathwa kwi-posterit fort I-Swab kufuneka ishiywe kwilungu lobufazi kangangemizuzwana eli-10 ukuya kweli-15 ukuyivumela ukuba ifumane imfihlakalo yobufazi. Tsala i-swab ngononophelo!.
■ Beka i-swab kwityhubhu yokukhupha, ukuba uvavanyo lungaqhutywa ngokukhawuleza. Ukuba uvavanyo kwangoko alunakwenzeka, iisampulu zesigulana kufuneka zibekwe kwityhubhu yothutho eyomileyo yokugcina okanye ukuthutha. I-Swabs inokugcinwa iiyure ezingama-24 kubushushu begumbi (15-30 ° C) okanye kwiveki e-4 nge-4 ° C okanye engaphezulu kwe-4 ° C okanye engaphezulu kwe-6 ° C. Zonke iimodeli kufuneka zivunyelwe ukufikelela kubushushu begumbi le-1-30 ° C ngaphambi kovavanyo.
Inkqubo
Yiza novavanyo, iisampuli, i-buffer kunye / okanye ulawulo kubushushu begumbi (15-30 ° C) ngaphambi kokusebenzisa.
■ Beka ityhubhu yokukhupha ecocekileyo kwindawo echongiweyo yomsebenzi. Yongeza i-1ml ye-buffer yokukhupha kwityhubhu yokukhupha.
■ Beka i-swimpaimen wible kwityhubhu. Ngamandla uxuba isisombululo ngokujikeleza i-swab ngamandla e-Tube kalishumi kalishumi (ngelixa unamanzi). Iziphumo ezilungileyo zifunyanwa xa i-curimen idityaniswa ngamandla kwisisombululo.
■ kutyibilika kulwelo oluninzi kangangoko kunokwenzeka kwi-swab ngokucinezela icala le-cube eguqukayo njengoko i-Swab isuswe. Ubuncinci i-1/2 yesisombululo se-buffer yesampulu kufuneka ihlale kwityhubhu yokufuduka eyaneleyo. Beka i-cap kwityhubhu ekhutshiweyo.
Lahla i-swab kwi-bioharser yenkunkuma efanelekileyo yenkunkuma.
■ I-capimes ekhutshiweyo inokugcina kubushushu begumbi kangangemizuzu engama-60 ngaphandle kokuchaphazela iziphumo zovavanyo.
■ Susa uvavanyo kwingxoxo yayo etywiniweyo, kwaye uyibeka kwindawo ecocekileyo nenomgangatho. Faka ilebhile ifowuni kunye nokuchongwa kwesigulana okanye ukulawula. Ukufumana isiphumo esifanelekileyo, i-assay kufuneka yenziwe ngaphakathi kwiyure enye.
■ Yongeza amaconsi ama-3 (malunga ne-100 μl) yesampulu ekhutshelweyo kwityhubhu yokukhupha kwisampulu yekhasethi yovavanyo.
Kulumkele ukubopha iibhubhu ze-tubs ze-air kwi-specian kakuhle (s), kwaye ungalahle nasiphi na isisombululo kwifestile yokujonga.
Njengoko uvavanyo luqala ukusebenza, uya kubona umbala uqhula ngaphaya kwe-membrane.
■ Lindela iqela lemibala (s) lokuvela. Isiphumo kufuneka sifundwe kwimizuzu emi-5. Musa ukutolika iziphumo emva kwemizuzu emi-5.
Lahla iityhubhu zovavanyo ezisetyenzisiweyo kunye neekhasethi zekhasethi zovavanyo kwi-bitser yenkunkuma efanelekileyo.
Ukuguquli kweziphumo
| IkhanyanisiIsiphumo:
| Iibhendi ezimbini ezinemibala zivela kwi-membrane. Ibhendi enye ivela kwingingqi yolawulo (c) kwaye enye ibhendi ivela kummandla wovavanyo (t). |
| EngalungangaIsiphumo:
| Iqela elinye kuphela elinemibala livela kwingingqi yolawulo (c). Akukho bandi zibonakalayo zibonakalayo kwingingqi yovavanyo (t). |
| AyisebenziIsiphumo:
| Ibhendi yolawulo iyasilela ukuvela. Iziphumo ezivela kulo naluphi na uvavanyo olungavelisi ibhendi yolawulo ngexesha lokufunda elichaziweyo kufuneka lilahlwe. Nceda ujonge inkqubo kwaye uphinde uphinde ngovavanyo olutsha. Ukuba ingxaki iyaqhubeka, ukuyeka ukusebenzisa i-kit kwangoko kwaye uqhagamshelane nomsasazi wakho wengingqi. |
PHAWULA:
1. Ubunzulu bombala kwingingqi yovavanyo (t) bungahluka ngokuxhomekeka kuxinzelelo lwezinto ezikhoyo ezikhoyo kwinqaku. Kodwa inqanaba lezinto ezinokubakho azinakugqitywa kolu vavanyo.
2. Ivolumu engonelanga, inkqubo yokusebenza engachanekanga, okanye yenza uvavanyo oluphelelwe lixesha zezona zizathu zibalulekileyo zokungaphumeleli kwebhendi.
ULAWULO LWEMEKO
■ Ulawulo lwenkqubo lwangaphakathi lubandakanyiwe kuvavanyo. Ibhendi enemibala evelayo kwingingqi yolawulo (c) ithathwa njengolawulo lwenkqubo yangaphakathi. Iqinisekisa ivolumu eyoneleyo kunye nenkqubo yenkqubo efanelekileyo.
■ Ulawulo lwenkqubo lwangaphandle lunokubonelelwa (ngesicelo kuphela) kwiikhithi ukuqinisekisa ukuba iimvavanyo zisebenza ngokufanelekileyo. Kananjalo, ulawulo lunokusetyenziselwa ukubonisa ukusebenza ngokufanelekileyo ngumsebenzi wovavanyo. Ukwenza uvavanyo olululo okanye olubi, gcwalisa amanyathelo kwiCandelo loVavanyo lweCandelo lokunyanga i-swab ngendlela efanayo njenge-swab.
UKUHLAZIYWA KOKUVAVANYA
1. Akukho kutoliko lobungakanani kufuneka lwenziwe ngokusekwe kwiziphumo zovavanyo.
2.Ungasebenzisi uvavanyo ukuba i-aluminium ye-aluminium ye-aluminium okanye amatywina engxowa engalunganga.
I-3.A inamandla®Isiphumo sovavanyo lweprofayile, nangona bebubona ubukho bolwelo lwe-Amniotic kwisampulu, ayifumani indawo yokuqhekeka.
I-Eleas ngazo zonke iimvavanyo zokuxilonga, iziphumo kufuneka zitolikwe ekukhanyeni kwezinye iziphumo zeklinikhi.
5.Ukunyuka kwe-membranes ye-membranes yenzekile kodwa ukuvuza kwamanzi amniotis kuphele kwiiyure ezili-12 ngaphambi kokuba kuthathwe iiyure ezili-12 ngaphambi kokuba kuthathwe i-projeet, i-Igfbp-1 inokubangela iziphumo ezibi.
Iimpawu zentsebenzo
Itheyibhuli: iqinile®Uvavanyo lwe-Prop vs. Olunye uvavanyo lwe-Brand
|
Imvakalelo yesihlobo: |
| Olunye uphawu |
| ||
| + | - | Iyonke | |||
| Amandla®IPROM Vavanyo | + | 63 | 3 | 66 | |
| - | 2 | 138 | I-140 | ||
|
| 65 | I-141 | 206 | ||
Imvakalelo yohlalutyo
Isixa esisezantsi esicokisekileyo se-Igfbp-1 kwimo ekhutshiweyo yi-12,5 μg / l.
Izinto eziphazamisayo
Inkathalo kufuneka ithathwe ukuba ingangcolisekisi okanye i-cervicovaginal okanye i-cirvicovaginal enee-lungisa, ii-soaps, iintsholongwane, okanye iikhrim. I-Lubristants okanye i-crims inokuthi iphazamise emzimbeni I-SOAPS okanye iintsholongwane zinokuphazamisana no-Antigder-Antign Reaction.
Izinto eziphazamisayo ezinokubakho zavavanywa ekugxinisweni ezinokufumaneka ngokuvakalayo kwi-Cervicovaginal. Ezi zinto zilandelayo aziphazamisi kwi-Assay xa ivavanywa kumanqanaba abolekileyo.
| Into | Uxinzelelo | Into | Uxinzelelo |
| Amppillin | 1.47 MG / ML | I-Prostaglandin F2 | I-0.033 mg / ml |
| Erythromycin | I-0.272 mg / ml | I-Prostaglandin E2 | I-0.033 mg / ml |
| Umchamo wasemamani 3rd Trimester | I-5% (i-vol) | I-MonOnstistatr (Mic Ponaole) | I-0.5 mg / ml |
| I-Oxetocin | 10 IU / ML | I-Indigo Carmine | I-0.232 mg / ml |
| I-Terbutaline | I-3.59 mg / ml | Inyani | I-0.849 mg / ml |
| DexomethaShasone | 2.50 mg / ml | I-Betadiner Gel | I-10 mg / ml |
| Mgso4•7h2o | 1.49 mg / ml | I-Betadiner Ciness | I-10 mg / ml |
| IRitodrine | I-0.33 mg / ml | K-yr jelly | I-62.5 mg / ml |
| I-Dermicolr 2000 | I-25.73 mg / ml |
Izalathiso zoncwadi
I-Erdemoglu naseMungan T. Ukubaluleka kokufumana izinto zokukhula kwe-insulin-1 kwi-Intanethi ekufikeleleni kwi-Cervicovaginal: Thelekisa uvavanyo lwe-nitrazine kunye ne-Amniotic Fluat. I-Acta obstet gynecol iskeda (2004) 83: 622-626.
I-KUBOTA T kunye ne-Daticachi H. Uvandlakanyo lokukhula kwe-Insulin-ngokungathi kukukhula kweprotein-1 njengesixhobo sokuxilonga sokuphuphuma kwe-membranes. J Obstet gyneol Der (1998) 24: 411-414.
I-rutanen em et al. Uvandlakanyo lovavanyo olukhawulezayo lwe-Insulin-njengeFaul Propein iprotein-1 ekufumaneni isifo se-membrane ehleliweyo. IClim Chim Arde (1996) 253: 91-101.
I-Rutaanen em, pekonen f, karkkainen t. umlinganiso we-insulin-njenge-insulin-1 kwi-Terving / i-Teaginal / Traines: Thelekisa i-rom-joysin ye-rom-joysines eqingqiweyo. IClim Thim Arde (1993) 214: 73-81.
Iglosari yesimboli
|
| Inombolo yekhathalogu |  | Ukunqongophala kobushushu |
 | Nxibelelana nemiyalelo yokusetyenziswa |
| Ikhowudi yeBatch |
 | Kwisixhobo sonyango lweVitrolostic |  | Sebenzisa |
 | Umvelisi |  | Iqulethwe ngokwaneleyo |
 | SUKUFUMANA |  | Ummeli ogunyazisiweyo kuluntu lwaseYurophu |
 | Imakishwe ngokwesixhobo sezonyango i-IVD 98/79 / EC | ||
