Uvavanyo lweNqaku
Ukusetyenziswa kwenjongo
I-strongstep®Uvavanyo lwe-CORTCINOnin luphawu olukhawulezileyo lwe-chromatographic ye-assomatographic ye-SEMICICT ye-SEMICTIONIT ye-CORTCINON kwi-PROURCINON kwi-serum yomntu okanye iplasma. Isetyenziselwa ukuchonga nokulawula unyango olunzima, intsholongwane kunye ne-seppis.
Intshayelelo
I-PREALCCONIN (PCT) yiproteni encinci equka intsalela ye-116 Amino Amino Acid enobunzima obuyi-13 kda eyathi yachazwa yi-moullec et al. Ngo-1984. I-PCT iveliswa ngokwesiqhelo kwiiseli ze-c-glands ze-thyroid. Ngo-1993, inqanaba eliphakamileyo le-PCT kwizigulana ezinosulelo lwe-bacrial yemvelaphi ye-bacrial yaxelwa kwaye i-PCT iqwalaselwe njengeyona nto iphawulekayo yokuphazamiseka kwenkqubo yenkqubo yokuvuza kunye ne-seppis. Ixabiso lokuxilonga le-PCT libalulekile ngenxa yonxibelelwano olusondeleyo phakathi koxinzelelo lwe-PCT kunye nobunzima bokuvusa. Yaboniswa ukuba "ukuvuvukala" i-pct ayiveliswanga kwiiseli ze-C. Iiseli zemvelaphi ye-neuroendornecrine zingumthombo we-PCT ngexesha losulelo.
Umgaqo
I-strongstep®I-CORTCITCININT STALCININT I-CREALT TOVICT TREALTS PROCTCITIN ngokutolikwa koMbala woPhuhliso lwangaphakathi. I-CORTALCINON MOOOCELNAl Antiby Ithangamelwanga kwingingqi yovavanyo ye-membrane. Ngexesha lovavanyo, i-projeks ephindaphindwayo nge-monoclonnika i-antiboscimonin antibodies idityaniswe kwibala elinemibala kwaye ichazwe kwi-conjugate pad yovavanyo. Umxube emva koko fudukela kwi-membrane ngezenzo ze-capillary kwaye inxibelelana nee-regents kwi-membrane. Ukuba kukho icebo elaneleyo kwi-Specialn, ibhendi enemibala iya kwenza kumda wovavanyo lwe-membrane. Ubukho beli band yebala libonisa isiphumo esihle, ngelixa ukungabikho kwayo kubonisa iziphumo ezimbi. Ukubonakala kweqela elinemibala kwingingqi yolawulo kusebenza njengokulawulwa kwenkqubo, ebonisa ukuba ivolumu efanelekileyo ye-Scamen kwaye iwikene ye-membrane yenzekile. Ukuphuhliswa kwemibala eyahlukileyo kummandla wovavanyo (T) Chaza isiphumo esifanelekileyo ngelixa isixa se-printalkonin sinokuvavanywa ngobungakanani bokubambisa umgca kwikhadi lokutolika. Ukungabikho komgca onombala kummandla wovavanyo (T)
iphakamisa isiphumo esibi.
UKULUMKELA
Le kit yenzelwe ukusetyenziswa kwe-vitro.
■ Le khit yeyokusetyenziswa kobungcali kuphela.
■ Funda imiyalelo ngononophelo ngaphambi kokuba wenze uvavanyo.
■ Le mveliso ayinazixhobo zomntu.
■ Musa ukusebenzisa iziqulatho zeKit emva komhla wokuphelelwa.
■ phatha zonke iisampulu njengoko usuleleke.
■ Landela inkqubo ye-LAB esemgangathweni kunye nezikhokelo ze-biosaflity zokuphatha kunye nokulahlwa kwezinto ezinokubakho. Xa inkqubo ye-Assay igqityiwe, ilahle imizekelo emva kokuziphindezela kwi-121 ℃ ubuncinci i-20 min. Ngenye indlela, banokunyangwa nge-0.5% ye-sodium hypochlorite iiyure ezininzi ngaphambi kokulahlwa.
■ Musa ukungasebenzi kakuhle ngomlomo kwaye kungabikho utshaya okanye utya i-assords.
■ hlawula iigloves ngexesha lenkqubo yonke.
