UKUSETYENZISWA OKUHLOSIWEYO
Inyathelo Eliqinileyo^®Uvavanyo lweProcalcitonin luvavanyo olukhawulezayo lwe-immune-chromatographic yokufumanisa i-semi-quantitative ye-Procalcitonin kwiserum yomntu okanye kwiplasma.Isetyenziselwa ukuxilonga kunye nokulawula unyango olunzima, usulelo lwebhaktheriya kunye ne-sepsis.

INTSHAYELELO
I-Procalcitonin (i-PCT) yiprotheni encinci equkethe i-116 i-amino acid intsalela kunye nobunzima be-molecular malunga ne-13 kDa eyaqala ukuchazwa nguMoullec et al.kwi-1984. I-PCT iveliswa ngokuqhelekileyo kwii-C-cell ze-thyroid glands.Kwi-1993, inqanaba eliphakamileyo le-PCT kwizigulane ezinosulelo lwenkqubo yemvelaphi yebhaktheriya kwabikwa kwaye i-PCT ngoku ithathwa njengeyona nto ibalulekileyo yokuphazamiseka ehamba kunye nokuvuvukala kwenkqubo kunye ne-sepsis.Ixabiso lokuxilonga le-PCT libalulekile ngenxa yobudlelwane obusondeleyo phakathi koxinzelelo lwe-PCT kunye nobukhulu bokuvuvukala.Kwaboniswa ukuba i-PCT "yokuvuvukala" ayiveliswanga kwiiseli ze-C.Iiseli zemvelaphi ye-neuroendocrine mhlawumbi zingumthombo we-PCT ngexesha lokuvuvukala.

UMGAQO
Inyathelo Eliqinileyo^®Uvavanyo olukhawulezayo lweProcalcitonin lufumanisa iProcalcitonin ngokutolika okubonakalayo kophuhliso lombala kumgca wangaphakathi.I-procalcitonin ye-monoclonal antibody ayishukumi kwindawo yovavanyo ye-membrane.Ngexesha lovavanyo, umzekelo uphendula kunye ne-monoclonal anti-Procalcitonin antibodies ezidityaniswe kumasuntswana anemibala kwaye zifakwe ngaphambili kwi-conjugate pad yovavanyo.Umxube ke ufuduka kwi-membrane ngesenzo se-capillary kwaye udibanise ne-reagents kwi-membrane.Ukuba kukho iProcalcitonin eyaneleyo kwi-specimen, ibhendi enemibala iya kwenza kwindawo yokuvavanya ye-membrane.Ubukho beli bhanti elinemibala libonisa umphumo omuhle, ngelixa ukungabikho kwawo kubonisa umphumo ombi.Ukubonakala kwebhanti enemibala kwindawo yokulawula isebenza njengolawulo lwenkqubo, ebonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.Uphuhliso lombala olwahlukileyo kumgca wovavanyo (T) lubonisa isiphumo esihle ngelixa isixa seProcalcitonin sinokuvavanywa ngokwesiqingatha sobungakanani ngokuthelekisa ubungakanani bomgca wovavanyo ukuya kugxininiso lomgca wereferensi kwikhadi lokutolika.Ukungabikho komgca onemibala kwingingqi yovavanyo (T)
ibonisa isiphumo esibi.

UKULUMKELA
Le khithi yeyokusetyenziswa kwe-IN VITRO yokuxilongwa kuphela.
■ Le khithi yeyokusetyenziswa NGOKWENGEZIWE kuphela.
■ Funda imiyalelo ngocoselelo phambi kokuba wenze uvavanyo.
■ Le mveliso ayinayo nayiphi na imathiriyeli yabantu.
■ Musa ukusebenzisa imixholo yekhithi emva komhla wokuphelelwa.
■ Bamba yonke imizekelo njengenokosulela.
■ Landela inkqubo yeLebhu esemgangathweni kunye nezikhokelo zokhuseleko lwebhayoloji ekuphathweni nasekulahlweni kwemathiriyeli enokuthi ibe yintsholongwane.Xa inkqubo yovavanyo igqityiwe, lahla iisampuli emva kokuzifakela i-autoclaving kwi-121℃ ubuncinane ubuncinane be-20 min.Kungenjalo, banokuphathwa nge-0.5% yeSodium Hypochlorite iiyure ngaphambi kokulahlwa.
∎ Musa i-pipette reagent ngomlomo kwaye ungatshayi okanye utyile ngelixa usenza iimvavanyo.
■ Nxiba iiglavu ngexesha lonke lenkqubo.