Uvavanyo lwe-HSV 12 yeAntigen
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INTSHAYELELO
I-HSV yimvulophu, i-DNA-equlethe intsholongwane ye-morphologically efana nenyeamalungu ohlobo Herpesviridae.Iintlobo ezimbini antigenically ezahlukeneyo ezieyaziwayo, uhlobo olukhethiweyo loku-1 kunye nohlobo lwesi-2.
Uhlobo lwe-HSV 1 kunye no-2 luhlala lubandakanyeka kusulelo oluphezulu lomlomoumgodi, ulusu, iliso kunye nelungu lobufazi, Usulelo lwemithambo-luvo esembindiniinkqubo (meningoencephalitis) kunye nosulelo olunzima oluqhelekileyo kumntwana osanda kuzalwaisigulana esine-immunocompromised nazo zibonwa, nangona kunqabile.Emva kweusulelo oluphambili lusonjululwe, intsholongwane inokubakho ngendlela efihlakeleyo kwixhalaizicubu, apho inokuphinda ivele khona, phantsi kweemeko ezithile, ibangele aukuphinda kweempawu.
Inkcazo yeklinikhi yeklasikhi ye-herpes yangasese iqala ngokusasazekaiimacules ezininzi ezibuhlungu kunye neepapules, ezithi ke zikhule zibe ngamaqela acacileyo,ii-vesicles ezizaliswe ngamanzi kunye neepustules.I-vesicles iyaphuka kwaye yenze izilonda.UlusuUqweqwe lwezilonda, ngelixa izilonda kwinwebu ye-mucous ziphilisa ngaphandle koqweqwe.Kwikwabasetyhini, izilonda zenzeka kwi-introitus, labia, perineum, okanye kwindawo ye-perianal.Amadodangokuqhelekileyo kuvela izilonda kwi-penial shaft okanye kwi-glans.Isigulane ngokuqhelekileyo siyakhulaithenda inguinal adenopathy.Usulelo lwePerianal luxhaphakile kwi-MSM.I-Pharyngitis inokukhula ngokuvezwa komlomo.
Uphando lweSerology lubonisa ukuba abantu abazizigidi ezingama-50 eUnited States banamalungu okuzalaUsulelo lwe-HSV.EYurophu, i-HSV-2 ifumaneka kwi-8-15% yabemi ngokubanzi.KwiAfrika, amazinga okuxhaphaka yi-40-50% kwi-20 yeminyaka ubudala.I-HSV yeyona iphambiliunobangela wezilonda zesini.Usulelo lwe-HSV-2 ubuncinane luphinda kabini umngcipheko wesondoUkufumana intsholongwane kaGawulayo (human immunodeficiency virus) kwaye iyanyukausulelo.
Kuze kube kutshanje, ukuhlukaniswa kwentsholongwane kwinkcubeko yeeseli kunye nokuzimisela kohlobo lwe-HSVkunye ne-fluorescent staining ibe yintsika yovavanyo lwe-herpes kwizigulanaukuveza amanxeba kumalungu obuni.Ngaphandle kovavanyo lwe-PCR ye-HSV DNAibonakaliswe ukuba inobuntununtunu kunenkcubeko yentsholongwane kwaye inento ethilengaphaya kwama-99.9%.Kodwa ezi ndlela kuqeqesho lweklinikhi okwangoku zilinganiselwe,ngenxa yokuba iindleko zovavanyo kunye nemfuneko yabanamava, abaqeqeshiweyoabasebenzi bezobugcisa ukwenza uvavanyo luthintele ukusetyenziswa kwabo.
Kukwakho novavanyo lwegazi olufumanekayo kurhwebo olusetyenziselwa ukukhangela udidiIzilwa-buhlungu ezithile ze-HSV, kodwa olu vavanyo lwe-serological alukwazi ukubona okokuqalausulelo ukuze zisetyenziswe kuphela ukulawula usulelo oluphinda luvele.Olu vavanyo lwe-antigen inoveli lunokwahlula ezinye izifo zesilonda sesini kunye namalungu angasesei-herpes, njengegcushuwa kunye ne-chancroid, ukunceda ukuxilongwa kwangaphambili kunye nonyangolosulelo lwe-HSV.
UMGAQO
IsiXhobo se-HSV antigen Rapid Test yenzelwe ukufumana i-antigen ye-HSVngokutolikwa okubonakalayo kophuhliso lombala kumgca wangaphakathi.Iinwebu yayingashukumi kunye ne-anti Herpes simplex virus monoclonal antibody on
ummandla wovavanyo.Ngethuba lovavanyo, umzekelo uvumelekile ukuba usabele ngombalai-monoclonal anti-HSV antibody colored partticals conjugates, ezaziqatywe ngaphambiliiphedi yesampula yovavanyo.Emva koko umxube uhamba kwi-membrane nge-capillary
isenzo, kwaye isebenzisana ne-reagents kwi-membrane.Ukuba bekukho HSV ngokwaneleyoi-antigens kwimizekelo, ibhendi enemibala iya kubumba kwindawo yovavanyo ye-membrane.Ubukho beli bhanti elinemibala libonisa umphumo omuhle, ngelixa ukungabikho kwawo kubonisa
isiphumo esibi.Ukubonakala kwebhanti enemibala kwindawo yokulawula isebenza njengeulawulo lwenkqubo.Oku kubonisa ukuba umthamo ofanelekileyo womfanekiso wongezwekwaye i-membrane wicking yenzekile.
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