UKUSETYENZISWA OKUHLOSIWEYO
Inyathelo Eliqinileyo^®Uvavanyo lweProcalcitonin luvavanyo olukhawulezayo lwe-immune-chromatographici-assay yokufumanisa i-semi-quantitative ye-Procalcitonin kwi-serum yabantu okanyeiplasma.Isetyenziselwa ukuxilonga kunye nokulawula unyango olunzima,usulelo lwebhaktheriya kunye ne-sepsis.

INTSHAYELELO
Iprocalcitonin (PCT) yiprotheni encinci equka iintsalela ze-amino acid eziyi-116.ngobunzima bemolekyuli obumalunga ne-13 kDa eyathi yachazwa okokuqalanguMoullec et al.ngowe-1984.I-PCT iveliswa ngokuqhelekileyo kwiiseli ze-C ze-thyroid gland.Ngowe-1993, iinqanaba eliphakamileyo le-PCT kwizigulane ezinosulelo lwenkqubo yemvelaphi yebhakteriakwaxelwa kwaye i-PCT ngoku ithathwa njengeyona nto ibalulekileyo yokuphazamisekaehamba kunye nokuvuvukala kwenkqubo kunye ne-sepsis.Ixabiso loxilongo lweI-PCT ibalulekile ngenxa yobudlelwane obusondeleyo phakathi koxinzelelo lwe-PCT kunyeubunzima bokukrala.Kwaboniswa ukuba i-PCT "yokuvuvukala" ayikhoiveliswe kwii-C-cell.Iiseli zemvelaphi ye-neuroendocrine mhlawumbi zingumthomboye-PCT ngexesha lokuvuvukala.

UMGAQO
Inyathelo Eliqinileyo^®Uvavanyo olukhawulezileyo lweProcalcitonin lubhaqa iProcalcitonin ngokubonwayoukutolikwa kophuhliso lombala kumgca wangaphakathi.ProcalcitoninI-antibody ye-monoclonal ayishukumi kwindawo yovavanyo yenwebu.Ngexeshauvavanyo, umzekelo uphendula kunye ne-monoclonal anti-Procalcitonin antibodiesidityaniswe kumasuntswana anemibala kwaye ifakwe ngaphambili kwi-conjugate pad yovavanyo.Umxube ke ufuduka kwi-membrane ngesenzo se-capillary kunyeisebenzisana ne-reagents kwi-membrane.Ukuba kukho iProcalcitonin eyaneleyo kwii-specimen, ibhendi enemibala iya kwenza kwindawo yokuvavanya ye-membrane.Iubukho beli bhanti enemibala ibonisa umphumo omuhle, ngelixa ukungabikho kwayoibonisa isiphumo esibi.Ukubonakala kwebhanti enemibala ekulawuleniummandla usebenza njengolawulo lwenkqubo, ebonisa ukuba umthamo ofanelekileyo weisampuli yongeziwe kwaye i-membrane wicking yenzekile.Uphuhliso lombala olwahlukileyo kwingingqi yovavanyo (T) lubonisa isiphumo esihlengelixa isixa seProcalcitonin sinokuvavanywa ngokulinganayo ngobuninziuthelekiso lobungakanani bomgca wovavanyo kugxininiso lomgca wereferensi kwiikhadi lokutolika.Ukungabikho komgca onemibala kwingingqi yovavanyo (T)ibonisa isiphumo esibi.

UKULUMKELA
Le khithi yeyokusetyenziswa kwe-IN VITRO yokuxilongwa kuphela.
■ Funda imiyalelo ngocoselelo phambi kokuba wenze uvavanyo.
■ Le mveliso ayinayo nayiphi na imathiriyeli yabantu.
■ Musa ukusebenzisa imixholo yekhithi emva komhla wokuphelelwa.
■ Bamba yonke imizekelo njengenokosulela.
■ Landela inkqubo yeLebhu eqhelekileyo kunye nezikhokelo zokhuseleko lwebhayoloji yokuphatha kunyeukulahlwa kwezinto ezinokuthi zichaphazeleke.Xa inkqubo yovavanyoGqibezela, lahla iisampulu emva kokuzilungisa ngokuzenzekela kwi-121℃ ubuncinane20 imiz.Kungenjalo, banokunyangwa nge-0.5% yeSodium Hypochloriteiiyure ngaphambi kokulahlwa.
∎ Musa i-pipette reagent ngomlomo kwaye ungatshayi okanye utyile ngelixa usenza iimvavanyo.
■ Nxiba iiglavu ngexesha lonke lenkqubo.