Ukusetyenziswa kwenjongo
I-strongstep^®Isixhobo sovavanyo lwe-Adenovirus (i-feed) luhlobo olukhawulezileyoUkungafumani i-Hounossitical yokufumanisa ukufumanisa i-adenovirus emntwiniIisampulu zefecal. Le kit yenzelwe ukusetyenziswa njengoncedo ekuxilongeni lwe-Adenovirus
usulelo.

Intshayelelo
I-Entensic Adenovirus, ikakhulu i-AD40 kunye ne-AD41, ingunobangela ophambili wesifo sohudoKubantwana abaninzi abaphethwe sisifo esibi seDiredi, okwesibinikuphela kwi-rotavirus. Isifo se-suaute sesayala sisifo esikhulu sokufaKuBantwana abancinci Ehlabathini lonke, ngakumbi kumazwe asaphuhlayo. AdenovirusI-pathogens yodwa kwihlabathi liphela, kwaye inokubangela urhudoKunyaka unyaka wonke. Usulelo lwenzeka rhoqo kubantwana ngaphantsiIminyaka emibini ubudala, kodwa ifunyenwe kwizigulana zayo yonke iminyaka.Izifundo zibonisa ukuba i-Adenovirus inxulumene ne-4-15% yazo zonkeamatyala asebhereni e-vistroenteritis.

Ikhawulezi kwaye ichanekile kwi-adenovirus-gestroenteritis ehambelana nayo iluncedoUkuseka i-Entintoloss ye-gastroenteritis kunye nolawulo lwesigulana esinxulumene noko.Ezinye iindlela zokuchonga ezifana ne-elektroni microscopy (em) kwayeI-nucleic acid hybridization iyabiza kwaye isebenza nzima. Xa ndinonikwa iUkuzimisela ukumisela ubume bosulelo lwe-adenovirus, kuyabiza kwayeIimvavanyo ezinzima kakhulu azinakuyimfuneko.

Umgaqo
Isixhobo sovavanyo lwe-Adenovirus (i-feed) ifumana i-AdenovirusNgokutolikwa okuqinisekileyo kophuhliso lwemibala kwingaphakathihlula. I-Anti-Adenovirus Antibodies ayifakwanga kwingingqi yovavanyo yei-membrane. Ngexesha lovavanyo, i-starimen iphendula nge-anti-Adenovirus antibodiesidityaniswe kwibala elinemibala kwaye icacisiwe kwiPad yePad yovavanyo.Umxube emva koko fudukela kwi-membrane ngesenzo se-capillary kwaye unxibelelanangeerejista kwi-membrane. Ukuba kukho i-adenovirus eyaneleyo kwinqaku, aIbhendi enemibala iya kwenza kwingingqi yovavanyo ye-membrane. Ubukho besiIbhendi enemibala ibonisa isiphumo esihle, ngelixa ukungabikho kwayo kubonakalisa ukungalungangaiziphumo. Ukubonakala kweqela lemibala kwingingqi yolawulo likhonza njengeUkulawulwa kwenkqubo, ebonisa ukuba ivolumu efanelekileyo ye-Specimenyongezwa kwaye iwikene ye-membrane yenzekile.

Inkqubo
Yiza iimvavanyo, iisampuli, i-buffer kunye / okanye ilawula kubushushu begumbi(15-30 ° C) ngaphambi kokusetyenziswa.
1. Ukuqokelelwa kokuqokelela kunye nonyango lwangaphambili:
1) Sebenzisa izikhongozeli ezicocekileyo, ezomileyo zengqokelela yengqokelela. Iziphumo ezilungileyo ziya kubaifunyenwe ukuba i-assay yenziwe ngaphakathi kweeyure ezi-6 emva kokuqokelela.
2) Kwimifanekiso eqinileyo: engayicothi kwaye ususe i-chabe ye-sublet tube. Yibangononophelo ukuba ungachithi okanye ispatter yesisombululo kwityhubhu. Qokelela iiSammelaNgokufaka i-Applicator inamathela kwiindawo ezi-3 ubuncinci zeI-feeds yokuqokelela i-50 mg yemitya (efanayo kwi-1/4 yepea).Imijelo yolwelo: Bamba ipipette ngokuthe nkqo, i-aspirate fecaliisampulu, emva koko ugqithisele amaconsi ama-2 (malunga ne-80 μl) kwiItyhubhu yengqokelela ye-Super equlethe i-buffer yokukhupha.
3) Guqula endaweni yabanini-sicelo ukuba babuye kwityhubhu kwaye bakhuphe i-cap. Yibangononophelo ukuba ungaphuli inqaku le-sube.
4) shake ityhubhu yengqokelela yamandla ukuxubana i-starmen kunyei-buffer yokukhupha. Iimpungutye ezilungiswe kwityhubhu yengqokelelaIngagcinwa kwiinyanga ezi-6 nge-20 ° C ukuba ayivavanywanga kwiyure enye emvaamalungiselelo.

2. Uvavanyo
1) Susa uvavanyo kwingxoxo yayo etywiniweyo, kwaye uyibekaumphezulu ococekileyo, wenqanaba. Ilebheli yovavanyo ngesigulana okanye ngolawuloisazisi. Kwiziphumo ezilungileyo, i-assay kufuneka yenziwe ngaphakathiyure.
2) Sebenzisa iqhekeza lephepha lezicubu, phula inqaku letyhubhu ye-dilution. BambelelaI-TUBE ngokuthe nkqo kwaye ihambisa i-3 yecophu yesisombululo kwi-piclen kakuhle(S) yesixhobo sovavanyo.Kulumkele ukubopha iibhubhu ze-air kwi-specimen kakuhle (s), kwaye akongezi
nasiphi na isisombululo kwifestile yesiphumo.Njengoko uvavanyo luqala ukusebenza, umbala uya kuthwala i-membrane.

3. Lindela iqela lemibala (s) lokuvela. Isiphumo kufuneka sifundwe nge-10imizuzu. Musa ukutolika iziphumo emva kwemizuzu engama-20.

Phawula:Ukuba i-timent ayifuduki ngenxa yobukho bamasuntswana, i-CentrifugeI-vilimes ekhutshiweyo equlethwe kwi-buffer vial vial. Qokelela i-100 μl yeI-Supernatantint, i-Dispensen kwi-Specimen Well STATA isixhobo sovavanyo kwaye siqale kwakhona, emva kwemiyalelo echazwe apha ngasentla.

Iziqinisekiso