UKUSETYENZISWA OKUHLOSIWEYO
Inyathelo Eliqinileyo^®I-Adenovirus Rapid Test Device (Iindle) ngumbono okhawulezayoI-immunoassay yokufumanisa umgangatho ophakamileyo we-adenovirus emntwiniimizekelo yelindle.Le kiti yenzelwe ukusetyenziswa njengoncedo ekuxilongweni kwe-adenovirus
usulelo.

INTSHAYELELO
I-Enteric adenoviruses, ikakhulu i-Ad40 kunye ne-Ad41, ngoyena nobangela uphambili worhudo.kubantwana abaninzi abanesifo sorhudo esiqatha, okwesibinikuphela kwii-rotaviruses.Isifo sorhudo singunobangela ophambili wokufakubantwana abancinci kwihlabathi jikelele, ngakumbi kumazwe asakhasayo.AdenovirusIintsholongwane ziye zabekwa zodwa kulo lonke ihlabathi, kwaye zinokubangela urhudoebantwaneni unyaka wonke.Usulelo lubonwa rhoqo kubantwana abangaphantsiiminyaka emibini ubudala, kodwa ifunyenwe kwizigulane zayo yonke iminyaka.Uphononongo lubonisa ukuba i-adenoviruses idibene ne-4-15% yazo zonkeamatyala esibhedlele se-viral gastroenteritis.

Ukuxilongwa ngokukhawuleza kunye nokuchanekileyo kwe-adenovirus-related gastroenteritis luncedoekusekeni i-etiology ye-gastroenteritis kunye nokulawulwa kwezigulane ezinxulumeneyo.Ezinye iindlela zokuxilonga ezifana ne-electron microscopy (EM) kunyeI-nucleic acid hybridization iyabiza kwaye ifuna umsebenzi.Xa ndinonikwa iUkuzinciphisa uhlobo losulelo lwe-adenovirus, olunjalo lubiza kwayeiimvavanyo ezinzima zabasebenzi zisenokungabi yimfuneko.

UMGAQO
I-Adenovirus Rapid Test Device (Iindle) ibona i-adenovirusngokutolikwa okubonakalayo kophuhliso lombala ngaphakathistrip.I-anti-adenovirus antibodies ayishukumi kwindawo yovavanyoinwebu.Ngexesha lokuvavanya, umzekelo uphendula kunye ne-anti-adenovirus antibodiesidityaniswe kumasuntswana anemibala kwaye ifakwe ngaphambili kwisampulu yesampula yovavanyo.Umxube ke ufuduka kwi-membrane ngesenzo se-capillary kwaye udibanisekunye ne-reagents kwi-membrane.Ukuba kukho i-adenovirus eyaneleyo kwi-specimen, aibhendi enemibala iya kwenza kwindawo yovavanyo ye-membrane.Ubukho bokuibhendi enemibala ibonisa umphumo omuhle, ngelixa ukungabikho kwayo kubonisa into embiisiphumo.Ukubonakala kwebhanti enemibala kwindawo yokulawula isebenza njengeulawulo lwenkqubo, ebonisa ukuba umthamo ofanelekileyo womzekelo uye wabayongezwa kwaye i-membrane wicking yenzekile.

INKQUBO
Zisa iimvavanyo, imizekelo, isithinteli kunye/okanye nolawulo kwiqondo lobushushu begumbi(15-30 ° C) ngaphambi kokusetyenziswa.
1. Ukuqokelelwa kwesampuli kunye nonyango lwangaphambili:
1) Sebenzisa izikhongozeli ezicocekileyo, ezomileyo xa uqokelela imizekelo.Iziphumo ezingcono kakhulu ziya kubaifunyenwe ukuba uvavanyo lwenziwa kwiiyure ezi-6 emva kokuqokelela.
2) Kwimizekelo eqinile: Khulula kwaye ususe i-applicator ye-tube dilution.YibaQaphela ukuba ungachithi okanye utshize isisombululo esivela kumbhobho.Qokelela imizekelongokufaka intonga yofakelo ubuncinane kwiziza ezi-3 ezahlukeneyo zeilindle lokuqokelela malunga ne-50 mg yelindle (elingana ne-1/4 ye-ertyisi).Kwimizekelo yolwelo: Bamba ipayipi ngokuthe nkqo, i-aspirate fecalimizekelo, kwaye emva koko udlulisele amathontsi ama-2 (malunga nama-80 µL)ityhubhu yokuqokelela umzekelo equlethe isithinteli sokutsalwa.
3) Buyisela i-applicator kwi-tube kwaye ujike i-cap ngokuqinileyo.Yibaqaphela ukuba ungaphuli incam yombhobho wokuxutywa.
4) Gubha i-tube yokuqokelela i-specimen ngamandla ukuxuba i-specimen kunyeisithinteli sokutsalwa.Iisampulu ezilungiselelwe kwityhubhu yokuqokelela isampuliinokugcinwa iinyanga ezi-6 kwi -20°C ukuba ayivavanywanga kwiyure enye emva kokoukulungiselela.

2. Uvavanyo
1) Susa uvavanyo kwisingxobo salo esitywiniweyo, kwaye usibekeindawo ecocekileyo, esemgangathweni.Bhala uvavanyo ngesigulane okanye ngolawuloukuchongwa.Ukuze ufumane iziphumo ezingcono, uvavanyo kufuneka lwenziwe ngaphakathiyure.
2) Ukusebenzisa isiqwenga sephepha le-tissue, phula incam yombhobho we-dilution.Bambaityhubhu ngokuthe nkqo kwaye usasaze amaconsi ama-3 esisombululo kwi-specimen kakuhle(S) yesixhobo sovavanyo.Kuphephe ukubambisa amaqamza omoya kwi-specimen kakuhle (S), kwaye ungafaki
Nasiphi na isisombululo kwifestile yesiphumo.Njengoko uvavanyo luqala ukusebenza, umbala uya kufuduka kwi-membrane.

3. Linda ukuba i(ama)bhanti enemibala ivele.Isiphumo kufuneka sifundwe ngo-10imizuzu.Musa ukutolika umphumo emva kwemizuzu engama-20.

Phawula:Ukuba i-specimen ayifuduki ngenxa yobukho bamaqhekeza, i-centrifugeimizekelo ekhutshiweyo equlethwe kwibhotile yesithinteli sokutsalwa.Qokelela i-100 µL yeamandla amakhulu, lahla kwisampulu yequla (S) lesixhobo esitsha sovavanyo kwaye uqale kwakhona, ulandela imiyalelo echazwe ngasentla.

Iziqinisekiso